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human lung adenocarcinoma cell lines calu 1  (ATCC)


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    Structured Review

    ATCC human lung adenocarcinoma cell lines calu 1
    Human Lung Adenocarcinoma Cell Lines Calu 1, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 650 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/calu+1+cell+lines/Calu-1/pm42173993-33-0-9
    Average 96 stars, based on 650 article reviews
    human lung adenocarcinoma cell lines calu 1 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Multi-Omics Characterization of Lactate-Associated Molecular Subtypes in Lung Cancer Suggests a Role for DKK1 in Lactate-Linked Migration, Invasion, and Lactylation Programs
    Article Snippet: .. A549 and Calu-1 cell lines (ATCC, Manassas, VA, USA) were cultured in RPMI-1640 medium (Gibco, NY, USA) supplemented with 10% FBS (Gibco, New York, NY, USA) and 1% penicillin-streptomycin at 37 °C in 5% CO 2 . ..

    Article Title: MA16.11 Early and Late Survival Comparison Between Oncological Versus Non-Oncological Patients Admitted to a General Intensive Care Unit in Chile
    Article Snippet: .. A549, HCC15 and Calu-1 cell lines obtained from ATCC were cultured in RPMI1640 containing 5% FBS, without antibiotics. ..

    Article Title: MA17.01 Cell Lineage and Chromatin Landscape of Lung Cancer Are Controlled by GATA6
    Article Snippet: .. A549, HCC15 and Calu-1 cell lines obtained from ATCC were cultured in RPMI1640 containing 5% FBS, without antibiotics. ..

    Article Title: Multi-Omics Characterization of Lactate-Associated Molecular Subtypes in Lung Cancer Suggests a Role for DKK1 in Lactate-Linked Migration, Invasion, and Lactylation Programs.
    Article Snippet: .. A549 and Calu-1 cell lines (ATCC, Manassas, VA, USA) were cultured in RPMI-1640 medium (Gibco, NY, USA) supplemented with 10% FBS (Gibco, New York, NY, USA) and 1% penicillin-streptomycin at 37 ◦C in 5% CO2. ..

    Article Title: Suppression of Sirt1 sensitizes lung cancer cells to WEE1 inhibitor MK-1775-induced DNA damage and apoptosis.
    Article Snippet: Lung cancer treatment remains a challenge for clinical practice and new therapeutic approaches are urgently needed.. Loss of functional WEE1 kinase causes DNA replication stress, DNA damage and unscheduled mitotic entry due to elevated CDK activity.. The selective WEE1 inhibitor MK-1775 synergize with DNA-damaging agent to inhibit cancer cell growth.

    other:

    Article Title: (+)-Usnic Acid Induces ROS-dependent Apoptosis via Inhibition of Mitochondria Respiratory Chain Complexes and Nrf2 Expression in Lung Squamous Cell Carcinoma
    Article Snippet: Human LUSC H520 and Calu-1 cell lines were originally from ATCC (Manassas, VA, USA).

    Incubation:

    Article Title: Tumoral NOX4 recruits M2 tumor-associated macrophages via ROS/PI3K signaling-dependent various cytokine production to promote NSCLC growth
    Article Snippet: .. A549 and Calu-1 cell lines, originally purchased from ATCC, were incubated at 37 °C in an atmosphere of 5% CO 2 in DMEM supplemented with 10% FBS, penicillin-streptomycin and glutamine (2 mM). .. Cells were transfected with 100 nM of a control shRNA, two individual shRNA against NOX4 or a pCMV-NOX4 cDNA plasmid with Lipofectamine 2000 (Invitrogen, 11668–019) overnight according to manufacturer's instructions according to our previous study .

    Transfection:

    Article Title: Suppression of Sirt1 sensitizes lung cancer cells to WEE1 inhibitor MK-1775-induced DNA damage and apoptosis.
    Article Snippet: Lung cancer treatment remains a challenge for clinical practice and new therapeutic approaches are urgently needed.. Loss of functional WEE1 kinase causes DNA replication stress, DNA damage and unscheduled mitotic entry due to elevated CDK activity.. The selective WEE1 inhibitor MK-1775 synergize with DNA-damaging agent to inhibit cancer cell growth.



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    Image Search Results


    Flowchart of the study illustrating the construction and analysis of the immune-related gene (IRG) prognostic signature for LUSC.

    Journal: Frontiers in Immunology

    Article Title: Identification of immune-related prognostic biomarkers in lung squamous cell carcinoma microenvironment

    doi: 10.3389/fimmu.2025.1724319

    Figure Lengend Snippet: Flowchart of the study illustrating the construction and analysis of the immune-related gene (IRG) prognostic signature for LUSC.

    Article Snippet: The human LUSC cell lines Calu-1 and NCI-H520, and the human monocytic cell line THP-1 were obtained from the American Type Culture Collection (ATCC).

    Techniques:

    Results of immune cell infiltration between high- and low-risk groups. (A, B) Histogram of immune cell infiltration. (C) Box plot of differential expression of 22 immune cells. (D) Proportion of 22 types of immune cells in LUSC patients. (E) Comparison of stromal cells in high-risk and low-risk groups. (F–L) The correlation between characteristic genes and immune cells. Significance levels indicated are based on Benjamini-Hochberg adjusted P -values (FDR). (*p < 0.05).

    Journal: Frontiers in Immunology

    Article Title: Identification of immune-related prognostic biomarkers in lung squamous cell carcinoma microenvironment

    doi: 10.3389/fimmu.2025.1724319

    Figure Lengend Snippet: Results of immune cell infiltration between high- and low-risk groups. (A, B) Histogram of immune cell infiltration. (C) Box plot of differential expression of 22 immune cells. (D) Proportion of 22 types of immune cells in LUSC patients. (E) Comparison of stromal cells in high-risk and low-risk groups. (F–L) The correlation between characteristic genes and immune cells. Significance levels indicated are based on Benjamini-Hochberg adjusted P -values (FDR). (*p < 0.05).

    Article Snippet: The human LUSC cell lines Calu-1 and NCI-H520, and the human monocytic cell line THP-1 were obtained from the American Type Culture Collection (ATCC).

    Techniques: Quantitative Proteomics, Comparison

    NR1D2 is downregulated in LUSC, inhibits tumor cell migration, invasion, and modulates the immune microenvironment. (A–D) Immunohistochemistry (A, C) and Western blot (B, D) analysis show significantly reduced NR1D2 expression in LUSC tissues compared to adjacent normal lung tissues (P < 0.001). Representative images (A, B) and quantification (C, D) are shown. (E–G) Efficient knockdown of NR1D2 in Calu1 and NCI-H520 cells using two independent siRNAs (si NR1D2 #1 and si NR1D2 #2) confirmed by Western blot (E, F) and qPCR (G) (P <0.001 vs. siCtrl). (H, I) Wound healing assays demonstrate significantly impaired migratory capacity in NR1D2 -silenced Calu1 and NCI-H520 cells. Representative images (H) at 0h and 24h and quantification of wound closure (I) are shown (P < 0.001 vs. siCtrl). (J–L) Transwell assays showing significantly reduced migration and invasion of Calu1 and NCI-H520 cell lines following NR1D2 silencing. Representative images (J) and quantification of migrated/invaded cells (K, L) are presented (P < 0.001 vs. siCtrl). (M–O) Flow cytometry analysis of THP-1-derived macrophages cultured in conditioned medium (CM) from NR1D2 -silenced tumor cells. Enhanced polarization towards CD206+CD163+ M2-like macrophages was observed. Representative dot plots (M) and quantification of CD206 + CD163 + macrophage following exposure to CM from NR1D2 -depleted Calu1 cells (N) and NCI-H520 cells (O) subsets are shown (**P < 0.001 vs. CM from siCtrl cells). (P, Q) ELISA assays show increased secretion of IL-10 (P) and TGF-β1 (Q) in macrophage supernatants following exposure to CM from NR1D2 -depleted tumor cells (*P < 0.05, **P < 0.01, ***P <0.001 vs. CM from siCtrl cells). All data are presented as mean ± SD. Scale bars are provided in the respective image panels.

    Journal: Frontiers in Immunology

    Article Title: Identification of immune-related prognostic biomarkers in lung squamous cell carcinoma microenvironment

    doi: 10.3389/fimmu.2025.1724319

    Figure Lengend Snippet: NR1D2 is downregulated in LUSC, inhibits tumor cell migration, invasion, and modulates the immune microenvironment. (A–D) Immunohistochemistry (A, C) and Western blot (B, D) analysis show significantly reduced NR1D2 expression in LUSC tissues compared to adjacent normal lung tissues (P < 0.001). Representative images (A, B) and quantification (C, D) are shown. (E–G) Efficient knockdown of NR1D2 in Calu1 and NCI-H520 cells using two independent siRNAs (si NR1D2 #1 and si NR1D2 #2) confirmed by Western blot (E, F) and qPCR (G) (P <0.001 vs. siCtrl). (H, I) Wound healing assays demonstrate significantly impaired migratory capacity in NR1D2 -silenced Calu1 and NCI-H520 cells. Representative images (H) at 0h and 24h and quantification of wound closure (I) are shown (P < 0.001 vs. siCtrl). (J–L) Transwell assays showing significantly reduced migration and invasion of Calu1 and NCI-H520 cell lines following NR1D2 silencing. Representative images (J) and quantification of migrated/invaded cells (K, L) are presented (P < 0.001 vs. siCtrl). (M–O) Flow cytometry analysis of THP-1-derived macrophages cultured in conditioned medium (CM) from NR1D2 -silenced tumor cells. Enhanced polarization towards CD206+CD163+ M2-like macrophages was observed. Representative dot plots (M) and quantification of CD206 + CD163 + macrophage following exposure to CM from NR1D2 -depleted Calu1 cells (N) and NCI-H520 cells (O) subsets are shown (**P < 0.001 vs. CM from siCtrl cells). (P, Q) ELISA assays show increased secretion of IL-10 (P) and TGF-β1 (Q) in macrophage supernatants following exposure to CM from NR1D2 -depleted tumor cells (*P < 0.05, **P < 0.01, ***P <0.001 vs. CM from siCtrl cells). All data are presented as mean ± SD. Scale bars are provided in the respective image panels.

    Article Snippet: The human LUSC cell lines Calu-1 and NCI-H520, and the human monocytic cell line THP-1 were obtained from the American Type Culture Collection (ATCC).

    Techniques: Migration, Immunohistochemistry, Western Blot, Expressing, Knockdown, Flow Cytometry, Derivative Assay, Cell Culture, Enzyme-linked Immunosorbent Assay